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To open the Insert sequence Restriction SIte dialog use the Edit ‣ Insert subsequence on Restriction SIteon sequence editing in the Actions main menu or in the context menu of the Sequence View or press the Ctrl-Shift-I key:

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Insert a sequence in the Paste data here field. Image Added

Select an enzyme, which site sequence you want to paste to the whole sequence in the "Choose site to paste" field.

The "Show sites with undefined suppliers" button makes visible all resriction enzimes avaliable. When it is unchecked (default) only enzymes, which have at leas one supplier, are available.

You can also configure the way how annotations located in an edited region should be modified.

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