This workflow allows This workflow allows to add PHRED quality scores to the sequence and save output to Fastq. For example, one can read a Fasta file, import PHRED quality values from corresponding qualities file and export the result to Fastq.
To execute the workflow do the following:
- Select "Sequence Reader" task and specify source file(s) at "URL" field in the Property Editor.
- Select "Import PHRED qualities" task and specify URL to the quality file. Usually such files have .qual extension.
- Launch the schema with pressing Ctrl+R shrotcut.
After running the workflow, target fastq file will appear in the same folder as the source file, with the same name but diffrent extension.
| Tip | ||
|---|---|---|
| ||
If you haven't used the workflow samples in UGENE before, look at the "How to Use Sample Workflows" section of the documentation. |
Workflow Sample Location
The workflow sample "Convert "seq/qual" Pair to FASTQ.
Workflow Image
The workflow looks as follows:
| HTML | ||
|---|---|---|
<center> <br> <img src="/wiki/download/attachments/324458916122704/Convert seq or -qual pairPair to FastqFASTQ.png"/> <br> </center> | ||
| Tip | ||
| ||
| If you haven't used the workflow samples in UGENE before, look at the "How to Use Sample Workflows" section of the documentation. |
Workflow Wizard
The wizard has 2 pages.
Input Sequence(s): On this page you must input sequences(s).
HTML <center> <br> <img src="/wiki/download/attachments/16122704/Convert seq-qual Pair to FASTQ_1.png"/> <br> </center>Convert "seq/qual" Pair to FASTQ: On this page you can modify converting settings.
HTML <center> <br> <img src="/wiki/download/attachments/16122704/Convert seq-qual Pair to FASTQ_2.png"/> <br> </center>