Workflow Designer Documentation v. 1.17.0
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  • Raw DNA-Seq Data Processing

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The wizard for single-end reads has 5 page.

  1. Input data: On  On this page tab you must need to input FASTQ file(s). the FASTQ files, obtained from the sequencer. One or several files can be specified as input.

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  <img src="/wiki/download/attachments/16122726/Raw DNA-Seq Processing_2.png"/>
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</center>

  2. Pre-processing: On this page you can modify filtration parameters.  On this tab there are parameters for sequencing reads trimming and the default file with adapter sequences that should be cut.

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  <img src="/wiki/download/attachments/16122726/Raw DNA-Seq Processing_3.png"/>
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    The following parameters are available:

    Quality threshold – trim all bases with quality score lower than the value of the parameter.

    Min length – the minimum length of a trimmed read. 

    Adapters – the list of adapter sequences to be trimmed by the cutadapt tool.


  3. Mapping: On this page you must input reference and optionally modify advanced parameters. 

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  <img src="/wiki/download/attachments/16122726/Raw DNA-Seq Processing_4.png"/>
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  4. Post-processing: On this page you can modify post-processing parameters. 

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  <img src="/wiki/download/attachments/16122726/Raw DNA-Seq Processing_5.png"/>
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  5. Output data: On this page you must input output parameters. 

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  <img src="/wiki/download/attachments/16122726/Raw DNA-Seq Processing_6.png"/>
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