Workflow Designer Documentation v. 1.17.0
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  • In Silico PCR

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  1. Input DNA Sequences: On this page you must input DNA sequences. 

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    <center>
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  <img src="/wiki/download/attachments/16123482/In Silico PCR Sample_1.png"/>
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</center>

  2. Primers and Parameters: Here you must input Primers and you can optionally modify In Silico PCR parameters.

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    <center>
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  <img src="/wiki/download/attachments/16123482/In Silico PCR Sample_2.png"/>
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    The following parameters are available:

    Primers URLA URL to the input file with primer pairs.
    MismatchesNumber of allowed mismatches.
    Min perfect matchNumber of bases that match exactly on 3' end of primers.
    Max product sizeMaximum size of amplified region.

  3. Output data: Here you can see information about output data.

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    <center>
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  <img src="/wiki/download/attachments/16123482/In Silico PCR Sample_3.png"/>
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