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Recalculate values of qualifiers - recalculates regions in qualifiers when sequence is modified.

Position to insert — the sequence position where to insert the subsequence.

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Annotated regions resolving —  modespecifies what to do with annotations that overlap with the region that is removed. You can select either Crop corresponding annotation (i.e. make it smaller) or Remove corresponding annotation.

Recalculate values of qualifiers - recalculates regions in qualifiers when sequence is modified.

Save resulted document to a new file — similar to the same parameter in the Insert subsequence dialog (described above).

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