Workflow Designer Documentation v. 1.20.0
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  • In Silico PCR

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This workflow simulates the PCR process.

Tip
titleHow to Use This Sample

If you haven't used the workflow samples in UGENE before, look at the "How to Use Sample Workflows" section of the documentation.

Workflow Sample Location

The workflow sample "In Silico PCR" can be found in the "Scenarios" section of the Workflow Designer samples.

Workflow Image

The opened workflow looks as follows:

HTML
<center>
  <br>
  <img src="/wiki/download/attachments/16123482/In Silico PCR Sample.png"/>
  <br> 
</center>
Workflow Wizard

The wizard has 3 pages.

  1. Input DNA Sequences: On this page you must input DNA sequences. 

    HTML
    <center>
  <br>
  <img src="/wiki/download/attachments/16123482/In Silico PCR Sample_1.png"/>
  <br> 
</center>

  2. Primers and Parameters: Here you must input Primers and you can optionally modify In Silico PCR parameters.

    HTML
    <center>
  <br>
  <img src="/wiki/download/attachments/16123482/In Silico PCR Sample_2.png"/>
  <br> 
</center>

    The following parameters are available:

    Primers URLA URL to the input file with primer pairs.
    MismatchesNumber of allowed mismatches.
    Min perfect matchNumber of bases that match exactly on 3' end of primers.
    Max product sizeMaximum size of amplified region.

  3. Output data: Here you can see information about output data.

    HTML
    <center>
  <br>
  <img src="/wiki/download/attachments/16123482/In Silico PCR Sample_3.png"/>
  <br> 
</center>