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- Length
- GC content
- Molecular weight
- Extinction coefficient
- Melting Tm
- nmole/OD260
- μg/OD260
GC content
TODO
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The percentage of guanine (G) and cytosine (C) bases in the sequence or its selected region, for example:
GC-content("ACGTAC") = ((0 + 1 + 1 + 0 + 0 + 1) / 6) * 100% = 50% |
If the sequence contains degenerate base characters, average values are used, for example:
GC-content("ACGNBCT") = ((0 + 1 + 1 + 1/2 + 2/3 + 1 + 0) / 7) * 100% ~= 59.52% |
In this example "1/2" is used for "N" (any nucleotide), "2/3" us used for "B" (that means "C", "G", or "T" according to the IUPAC notation).
Molecular weightsad
TODO
Extinction coefficient
TODO
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