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- From NCBI or ENSEMBLE database
- Align the genomic and cDNA sequences using spliced aligner
Options
Exon annotation name
Span exon-exon junction
Exon rangeTo use this feture go to the RT-PCR tab of the Primer Designer dilaog:
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The following parameters are available:
Exon annotation name - exon annotation name.
Span exon-exon junction - minimum exon junction overlap size.
Exon range - exon range.
Span at least one intron: the default mode.
Max numbers of pairs to query - max numbers of pairs to query.