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Statistics about an opened sequence can be found on the Statistics tab of the Options Panel. When a region is selected in the sequence, the statistics is are interactively re-calculated for this region only. The following information is available:

  • Common statistics (length, molecular weight, etc.) - see the detailed description below
  • Characters occurrence
  • Dinucleotides occurrence - available for nucleotide sequences only
  • Codons occurrence - available for nucleotide sequences only
  • Amino  acids occurrence - available for nucleotide sequences only

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where fAT and fGC are fractions of AT and GC base pairs, respectively.

Melting temperature

The melting temperature is calculated as follows. For sequences of length 15 or longer:

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Tm 64.9 + 41 * (nG + nC - 16.4) / (nA + nT + nG + nC)

For shorter sequences:

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Tm = (nA + nT) * 2 + (nG + nC) * 4

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Click on the gear icon Image Addednear Melting temperature label and the Melting temperature settings dialog will be opened.
Note, that  UGENE computes Tm fot sequence regions from 8 to 10000 bp. Otherwise, an undefined value N/A is output.

See the corresponding page for settings details.

nmole/OD260

The amount of DNA or RNA represented in nanomoles per 1 unit of absorbance at 260 nm dissolved in 1 ml cuvette with 1 cm pathlength.

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