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  <img src="/wiki/download/attachments/1612189065930523/Trim and Align Sanger Reads.png"/>
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1. Reference Sequence: On this page you must input reference sequence.  

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   <center> <br> <img src="/wiki/download/attachments/16122743/Trim and Align Sanger Reads_1.png"/> <br> </center>

2. Input Sanger Reads (ABI Files): On this page you must input ABI file(s).  

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   <center> <br> <img src="/wiki/download/attachments/16122743/Trim and Align Sanger Reads_2.png"/> <br> </center>

3. Trimming and Filtering: On this page you can modify trimming and filtering settings.  

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   <center> <br> <img src="/wiki/download/attachments/16122743/Trim and Align Sanger Reads_3.png"/> <br> </center>

   The following parameters are available:

   Quality threshold	Quality threshold for trimming.
   Min Length	Too short reads are discarded by the filter.

4. Results: On this page you can modify output files settings.  

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   <center> <br> <img src="/wiki/download/attachments/16122743/Trim and Align Sanger Reads_4.png"/> <br> </center>