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  • A - the pair of primers closest to the amplified fragment, corresponding to the entire set of parameters.
  • B1 -
  •  the
  • the pair of primers closest to the amplified fragment, corresponding to the entire set of parameters
  • ,
  • if B2 and/or B3 exist.
  • Could be the same as A, but not always.
  • B2 - the pair of primers corresponding to the entire set of parameters, which has a 4-nucleotide intersection with B1.
  • B3 -
  •  the
  • the pair of primers corresponding to the entire set of parameters, which starts immediately when B1 ends.

The example of a forward primers result.

Also, you may see results in the result table. Single A single click on the result in the result table select selects the corresponding annotation, double click - export the result resulting primer to the separate file (with the backbone sequence, which will be added during the exporting process).

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