Child pages
  • Information about Sequence

Versions Compared

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

...

  • Common statistics
    • Length - number of bases in the analyzed sequence
    • GC content - the molar percentage of guanine and cytosine bases in an oligonucleotide sequence
    • Molar weight - is the sum of the atomic masses of the constituent atoms for 1 mole of oligonucleotide
    • Molat ext. coefficient - the molar extinction coefficient is a physical constant that is unique for each sequence and describes the amount of absorbance at 260nm (A260) of 1 mole/L DNA solution measured in 1 cm path-length cuvette
    • Melting TM - melting temperature is the temperature at which an oligonucleotide duplex is 50% in single-stranded form and 50% in double-stranded form.
    • nmole/OD260 - the amount of oligonucleotide in nanomoles that, when dissolved in 1 mL volume, results in 1 unit of absorbance at 260 nm with a standard 1 cm path-length cuvette
    • μg/OD260 - the amount of oligonucleotide in micrograms that, when dissolved in 1 mL volume, results in 1 unit of absorbance at 260 nm with a standard 1 cm path-length cuvette
  • Characters occurrence
  • Dinucleotides occurrence (for sequences with the standard DNA and RNA alphabets)

...