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<center> <br> <img src="/wiki/download/attachments/1612242416125631/In Silico PCR.png"/> </br> </center> |
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Maximum product - maximum size of the amplified sequence.
Extract annotations - specify the type of extracted annotations: Inner, All intersected or None.
- Value Inner is selected by default. When this value is selected, the extracted PCR product contains annotations from the original sequence, located within the extracted region.
- Value All intersected specifies that all annotations of the original sequence that intersect the extracted region must be extracted as well.
- Value None specifies that annotations from the original sequence must not be extracted.
Choosing primers
Type two primers for running In Silico PCR. If the primers pair is invalid for running the PCR process then the warning is shown. Also, primers for the running In silico PCR can be chosen from a primer library. Click the following button to choose a primer from the primers library:
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<center> <br> <img src="/wiki/download/attachments/1343507316125631/PrimersIn Silico LibraryPCR_12.png"/> </br> </center> |
The table consists of the following columns: name, GC-content (%), Tm, Length (bp) and sequence. Select primer in the table and click the Choose button.
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