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  • Making Request to Database

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  • If you’re using BLAST select the Analyze ‣ Query with BLAST item in the context menu or in the Actions main menu.
  • If you’re using BLAST+ select the Analyze ‣ Query with BLAST+ item in the context menu or in the Actions main  the main menu.

The Request to local BLAST database dialog will appear:

HTML
<center>
     <br>
     <img src="/wiki/download/attachments/1975971922056086/Making Request to Database.png"/>
     <br>
</center>

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Select search - here you should select the tool you would like to use. If the query sequence is a nucleotide sequence then blastnblastx and tblastx items are available. For a protein sequence, the items are blastp and tblastn.

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Culling limit - the maximum number of hits that will be shown (not equal to a number of annotations). The maximum availablle available number is 5000.

Search for short, nearly exact matches - automatically adjusts the word size and other parameters to improve results for short queries.

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Base name for BLAST DB files - the base name for the BLAST database files.

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