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Annotated regions resolving mode — defines either to Expand affected annotationRemove affected annotation, Split (join annotation parts) or Split (separate annotation parts) in case when the subsequence is inserted to the sequence position where some annotations are presented.

Recalculate values of qualifiers - recalculates regions in qualifiers when sequence is modified.

Position to insert — the sequence position where to insert the subsequence.

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In case a subsequence has been selected, the Replace subsequence is available from the context menu or by the Ctrl+R shortcut. The dialog opened in this case is similar to the dialog described above, except it already contains the sequence to be edited an doesn’t allow to input the start position.

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Also it is possible to remove selected subsequence from a sequence. When you select corresponding item (in the context menu or in the Actions menu), the Remove subsequence dialog appears:

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Description of the parameters:

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Annotated regions resolving —  modespecifies what to do with annotations that overlap with the region that is removed. You can select either Crop corresponding annotation (i.e. make it smaller) or Remove corresponding annotation.

Recalculate values of qualifiers - recalculates regions in qualifiers when sequence is modified.

Save resulted document to a new file — similar to the same parameter in the Insert subsequence dialog (described above).

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