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From this chapter you can learn how to search for Open Reading Frames (ORF) in a DNA sequence. The ORFs found are stored as automatic annotations. This means that if the automatic annotations highlighting has been enabled then ORFs are searched and highlighted for each sequence opened. Refer Automatic Annotations Highlighting to learn more.

To open the ORF Marker dialog, select the Analyze ‣ Find ORFs item in the context menu.

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When the search parameters has been selected and the OK button has been pressed in the dialog, the auto-annotating becomes enabled. In the Annotations editor the ORFs annotations can be found in the Auto-annotations\orf group.

After the search has been finished you can browse the results, sort them by length, strand or start position and save as annotations to the original sequence in the Genbank format.

For more information about codons use the codon table. It depends on the translation code selected for the sequence. To show or hide the table use Ctrl+T shortcut or click the Show codon table submenu of the Amino translation toolbar button menu:

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The codon table will appear:

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Clicking on a codon name redirects you to Wikipedia to give you a brief description of the corresponding amino acid. Cells of the table are colored according to classes of amino acids.