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  • Bowtie Aligning Short Reads

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Result file name — file in SAM format to write the result of the alignment into. This parameter is required.

Library - single-end or paired-end reads.

Prebuilt index — check this box to use an index file instead of a source reference sequence. The index is a set of 6 files with suffixes .1.ebwt, .2.ebwt, .3.ebwt, .4.ebwt, .rev.1.ebwt, and .rev.2.ebwt. The index is created during the alignment. Also you can build it manually.

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Note

Short reads length for Bowtie can’t be more than 1024.

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Seed (–seed) — pseudo-random number generator.Threads — launch the specified number of parallel search threads. Threads will run on separate processors/cores and synchronize when parsing reads and outputting alignments.

The following flags are available:

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