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The Remote BLAST plugin provides the a capability to annotate sequences with information stored in the NCBI BLAST remote database.

To perform a remote database search open the Sequence View, select the a sequence region to analyze and click the Analyze ‣ Query remote NCBI BLAST database context menu item. If a region is not selected the whole sequence will be analyzed.

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You can see the description of the annotation saving parameters here.

Search timeout — sometimes a database doesn’t respond, therefore you need to re-wait for the response. This option sets the time that will be spent for re-appeal to the database. Note that in case of long sequences time for request preparation increases and the search takes several minutes.the remote task terminated if the timeout is reached.

Tip

There is a little difference in default values of parameters between NCBI Nucleotide BLAST web interface and UGENE:

  • The web interface uses the megablast option by default: the search is fast, but only highly similar sequences are found.
  • UGENE ignores the option by default: the search may take more time, but all somewhat similar sequences are found.

Check the Megablast option, if you want exactly the same results to be found in UGENE as you had in the NCBI web interface.

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The Advanced options tab is not available when the cdd search is selected.

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