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BWA is a fast light-weighted tool that aligns relatively short reads to a reference sequence. Click this link to open BWA homepage. BWA-MEM is generally recommended for high-quality queries as it is faster and more accurate. BWA-MEM also has better performance than BWA-backtrack for 70-100bp Illumina reads.

Open Tools ‣ Align to reference submenu of the main menu.

     <img src="/wiki/download/attachments/10289161/BWA-MEM.png"/>

Select the Align short reads item to align short reads to a DNA sequence using BWA-MEM. Or select the Build index item to build an index for a DNA sequence which can be used to optimize aligning of short reads.

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