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Primers for the running In Silico PCR can be chosen from a primers library. Primers library it The primer library is a storage for keeping user primers. The added primers are stored between UGENE sessions.

To choose a primer from the primers library click on the following button:

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  <img src="/wiki/download/attachments/13435073/Primers Library.png"/>
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The following dialog will appear:

Go to the Tools->Primer->Primer library context menu to configure the primer library. The following window will appear: 

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  <img src="/wiki/download/attachments/13435073/Primers Library_12.png"/>
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The table consists of the following columns: name, GC-content (%), Tm, Length (bp) and sequence. Select primer in the table and click on the Choose button. 

To configure primers library go to the Tools->Primer->Primer library context menu. The following window will appear:

Click the New primer button to add a new primer. The following dialog will appear: 

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  <img src="/wiki/download/attachments/13435073/Primers Library_23.png"/>
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Input the primer sequence and primer name and click the OK button.

Select

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the primer in the table (you can use Ctrl and Shift) and

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click Remove primer(s) button to remove primer(s). 

Select the primer and click the Edit primer button to edit primer.