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3' perfect match - specify the number of nucleotides at the 3' end that do must not have mismatches.
Maximum product - maximum size of the amplified sequence.
Choosing primers
Type two primers for running In Silico PCR. If the primers pair is invalid for running the PCR process then the warning is shown. Also, primers for the running In silico PCR can be chosen from a primers primer library. Click the following button to choose a primer from the primers library:
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