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The workflow sample, described below, allows one to extract a coverage and/or bases count from an assembly. It receives a number of assemblies and for each of them produces coverage as a tab delimited plain text file. The coverage is extracted considering a threshold value.

Tip
titleHow to Use This Sample

If you haven't used the workflow samples in UGENE before, look at the "How to Use Sample Workflows" section of the documentation.

Workflow Sample Location

The workflow sample "Extract Coverage from Assembly" can be found in the "NGS" section of the Workflow Designer samples.

Workflow Image

The opened workflow looks as follows:

HTML
<center>
  <br>
  <img src="/wiki/download/attachments/13008925/Extract Coverage from Assembly.png"/>
  <br> 
</center>
Workflow Wizard

The wizard has 3 pages.

  1. Input assembly (-ies) Page: On this page you must input assembly(-ies). 

    HTML
    <center>
      <br>
      <img src="/wiki/download/attachments/13008925/Extract Coverage from Assembly_1.png"/>
      <br> 
    </center>

     

  2. Extract parameters Page: Here you can optionally modify extract parameters.

    HTML
    <center>
      <br>
      <img src="/wiki/download/attachments/13008925/Extract Coverage from Assembly_2.png"/>
      <br> 
    </center>

     The following parameters are available:

    FormatFormat to store the output.
    ExportData type to export.
    ThresholdThe minimum coverage value to export.
  3. Output data Page: On this page you can select an output file:

    HTML
    <center>
      <br>
      <img src="/wiki/download/attachments/13008925/Extract Coverage from Assembly_3.png"/>
      <br> 
    </center>