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  • Searching for TFBS with Weight Matrices

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Comment: Migration of unmigrated content due to installation of a new plugin

Task Name: pwm-search

Searches for transcription factor binding sites (TFBS) with position weight matrices (PWM) and saves the regions found as annotations.

Parameters:

seq — semicolon-separated list of input sequence files to search TFBS in. [String, Required]

matrix — semicolon-separated list of the input PWM. [String, Required]

out — output Genbank file.

name — name of the annotated regions. [String, Optional, Default: “misc_feature”]

min-score — minimum percentage score to detect TFBS. [Number, Optional, Default: 85]

strand — strands to search in. [Number, Optional, Default: 0]

The following values are available:

    • 0 (both strands)
    • 1 (direct strand)
    • -1 (complement strand)

Example:

Code Block
ugene pwm-search --seq=input.fa --matrix=Aro80.pwm;Aft1.pwm --out=res.gb