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UGENE provides GUI for a lot of different bioinformatics tools. A tool is supplied either as embedded or external:

  • Embedded tool: the tool is integrated into the UGENE source code. Such tools are always available in UGENE independently on the UGENE package type and operating system. 
  • External tool: the tool executable file (or files) is provided with UGENE. It is launched when one uses the tool in the UGENE GUI. The result is also shown in the UGENE GUI. Note that the tool may be available on some operating systems only.

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  1.  Download the tools executables, see links on the "Download UGENE and components" page:
    • External Tool Tools Package: the package contains all external tools except JRE (i.e. "java") and RScript.
    • Java Runtime Environment (JRE): although UGENE is not a Java application, a few tools (e.g. FastQC, Trimmomatic) require JRE. Make sure JRE 8 or higher is installed.
    • RScript: R is currently used in UGENE for ChIP-Seq data analysis only.

  2. Configure the tools in UGENE: open the UGENE Application Settings dialog, select the "External Tools" page and specify the external tools executables.

Integrated external tools

The following external tools are currently integrated into UGENE:See the list of the currently integrated external tools, information about their availability on different operating systems and version included into the corresponding External Tools Package in the table below.

                     PhyML            
External toolWindows (64-bit)Windows (32-bit)macOS (64-bit)Linux (64-bitWindows 32-bit)Linux (32-bit)
bedGraphToBigWig4    
bedtools2.20.1    
BLAST2.2.26    
BLAST+2.2.30    
Bowtie0.12.7    
Bowtie22.1.0    
BWA0.7.12    
CAP312/21/07    
CEASTODO    
CLARKN/A    
ClustalO1.2.1    
ClustalW2.1    
ConductGOTODO    
conservation_plotTODO    
Cufflinks toolsN/A    
Cutadapt1.7.1    
DIAMONDN/A    
FastQC0.11.2     
JRE (java)JRE is not included into the External Tools Packages.
HMMER3.1b2    
KrakenN/A    
MACS1.4.2    
MAFFT7.212    
MrBayes3.2.3    
peak2gene1.02    
perl5.10.0.5 (32-bit)    
peak2genePhyML20120412    
python2.7.5     
RscriptR is not included into the External Tools Packages.
samtools0.1.19    
SeqPosTODO    
SnpEff4.3i    
SPAdesN/A    
SpideyVersion not specified    
StringTieN/A    
T-Coffee8.99    
TopHatN/A    
Trimmomatic0.38    
vcftools0.1.12b    
WEVOTEN/A