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Finds annotations for the supplied DNA sequence in the NCBI remote database.

Element type: blast

Parameters

in GUI

ParameterDescriptionDefault valueParameter in Workflow FileType
Database Selects the database to search through. Available databases are blastn, blastp and cdd.ncbi-blastndb

string

Available values are:

  • ncbi-blastn
  • ncbi-blastp
  • ncbi-cdd
DatabaseSelect the database to search through.
db
 
string
Expected valueThis parameter specifies the statistical significance threshold of reporting matches against the database sequences.10e-valstring
Results limitThe maximum number of results.10hitsnumeric
MegablastUse megablast.Falsemegablastboolean
Short sequenceOptimizes search for short sequences.Falseshort-sequenceboolean
Entrez queryEnter an Entrez query to limit search.
entrez-query
 
string
Annotate asName of the result annotations.
result-name
 
string
BLAST outputLocation of the BLAST output file. This parameter insignificant for cdd search.
blast-output
 
string
Gap costsCost to create and extend a gap in an alignment.2 2gap-costsstring
Match scoresReward and penalty for matching and mismatching bases.1 -3

Parameters in Workflow File

Type: blast-ncbi

ParameterParameter in the GUITypedbDatabase

string

Available values are:

ncbi-blastndbDatabasestringe-valExpected valuestringhitsResults limitnumericmegablastMegablastbooleanshort-sequenceShort sequencebooleanentrez-queryEntrez querystringresult-nameAnnotate asstringblast-outputBLAST outputstringgap-costsGap costsstring
  • ncbi-blastp
  • ncbi-cdd
  • match-
    scoresMatch
    scoresstring


    Input/Output Ports

    The element has 1 input port:

    Name in GUI: Input sequence

    Name in Workflow File: in-sequence

    Slots:

    Slot In GUISlot in Workflow FileType
    Sequencesequencesequence

    And 1 output port:

    Name in GUI: Annotations

    Name in Workflow File: out-annotations

    Slots:

    Slot In GUISlot in Workflow FileType
    Set of annotationsannotationsannotation-table