...
- Length
- GC content
- Molecular weight
- Extinction coefficient
- Melting Tm
- nmole/OD260
- μg/OD260
GC content
TODO: add description/meaning?
The percentage of guanine (G) and cytosine (C) bases in the sequence or its selected region, for example:
...
In this example "1/2" is used for "N" (any nucleotide), "2/3" us used for "B" (that means "C", "G", or "T" according to the IUPAC notation).
Molecular weight
TODO: add description/meaning?
Molecular weight for a single-stranded molecule is calculated as the sum of the atomic masses of the molecule compounds:
DNA molecular weight = nA*251.24 + nT*242.23 + nC*227.22 + nG*267.24 + (n-1)*62 RNA molecular weight = nA*267.24 + nU*244.20 + nC*243.22 + nG*283.24 + (n-1)*62 |
Here "nA", "nT", "nC", "nG", "nU" denote the number of the corresponding nucleotide in the molecule, "n" is the number of all bases (62 is the weight of an internal phosphate).
Note that for degenerate base characters average value of nucleotide weight is used, for example, if the sequence also contain "Y" characters (that is "C" or "T"), the sum will include one more summand - "nY*(242.23 + 227.22)/2".
Molecular weight for a double-stranded molecule is calculated as the sum of the single strands molecular weights.
Extinction coefficient
TODO
Context information about a sequence can be found on the All information is contextual, i.e. it shows statistics about the currently selected region (on the selected sequence). The tab includes information about:
...