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By default, a phylogenetic tree is synchronized with the corresponding alignment.

To disable/enable synchronization use the Tree and Alignment synchronization button. Image Added

To load a tree from a file follow the instruction described in the Opening Document paragraph or use the Tree settings tab of the Options Panel. For example, you may open the $UGENE\data\samples\Newick\COI.nwk sample file provided within UGENE package.

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