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  1. Reference Sequence: On this page you must input reference sequence.  

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  2. Input Sanger Reads (ABI Files): On this page you must input ABI file(s).  
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  3. Trimming and FilteringMapping Settings: On this page, you can modify trimming and filtering mapping settings.  
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    The following parameters are available:

    Quality Trimming quality thresholdQuality The quality threshold for trimming.Min LengthToo short reads are discarded by the filter.
    Mapping min similarity

    Reads,whosesimilaritywiththereferenceislessthanthestatedvalue,willbeignored.

    Read name in the resulting alignment

    Reads in the resulting alignment can be named either by the names of the sequences in the input files or by the input files names. For example, if the sequences have the same name, set this value to &quot; File name&quot; to be able to distinguish the reads in the resulting alignment.

  4. Results: On this page you can modify output files settings.  

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