Page tree

Versions Compared

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

You can build an index to optimize short reads alignment using UGENE Genome Aligner. To open the Build Index dialog, select the Tools ‣ NGS data analysis ‣ Build index for reads mapping item in the main menu. Set value of the Align the Map short reads method parameter to UGENE Genome Aligner.

...

HTML
<center>
     <br>
     <img src="/wiki/download/attachments/422772365930893/Building Index for UGENE Genome Aligner.png"/>
     <br>
</center>

...

Reference fragmentation — this parameter influences the amount of parts the reference will be devideddivided. It is better to make it bigger, but it influences the amount of memory used during the alignment.

...