To associate variations with the assembly, open the sequence (the sequence must be loaded) and drag it to the Assembly Reference Area:
![](/wiki/download/attachments/4227538/Associating Reference Sequence.png)
The sequence appears in the Reference Area:
![](/wiki/download/attachments/4227538/Associating Reference Sequence_1.png)
To remove the association, select the Unassociate item in the Reference Area context menu.