Primers for the running In Silico PCR can be chosen from a primers library. Primers library it is a storage for keeping user primers. The added primers are stored between UGENE sessions.

To choose a primer from the primers library click on the following button:

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  <img src="/wiki/download/attachments/13435073/Primers Library.png"/>
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The following dialog will appear:

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  <img src="/wiki/download/attachments/13435073/Primers Library_1.png"/>
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The table consists of the following columns: name, GC-content (%), Tm, Length (bp) and sequence. Select primer in the table and click on the Choose button. 

To configure primers library go to the Tools->Primer->Primer library context menu. The following window will appear:

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  <img src="/wiki/download/attachments/13435073/Primers Library_2.png"/>
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• The table columns are: name, …
• Click “New Primer” for adding a new one.
• Select primers in the table (you can use Ctrl and Shift) and then click “Remove” for removing the selected primers from the library.