The workflow sample, described below, prepare ChIP-Seq processed data (with BedTools and bedGraphToBigWig) for visualization in a genome browser. For input BED-file produces BigWig file.
If you haven't used the workflow samples in UGENE before, look at the "How to Use Sample Workflows" section of the documentation. |
The workflow sample "ChIP-Seq Coverage" can be found in the "NGS" section of the Workflow Designer samples.
The opened workflow looks as follows:
<center> <br> <img src="/wiki/download/attachments/13008919/Chip-Seq Coverage.png"/> <br> </center> |
The wizard has 3 pages.
Input data Page: On this page you must input BED file with ChIP-Seq tags.
<center> <br> <img src="/wiki/download/attachments/13008919/Chip-Seq Coverage_1.png"/> <br> </center> |
Parameters Page: Here you can optionally modify parameters that should be used for the Slopbed, Genome Coverage and BedGraphToBigWig elements.
<center> <br> <img src="/wiki/download/attachments/13008919/Chip-Seq Coverage_2.png"/> <br> </center> |
Output Files Page: On this page you can select an output directory:
<center> <br> <img src="/wiki/download/attachments/13008919/Chip-Seq Coverage_3.png"/> <br> </center> |