This workflow filters sequences with quality >= than parameter "quality" and writes result in file in FASTQ format.

If you haven't used the workflow samples in UGENE before, look at the "How to Use Sample Workflows" section of the documentation.

Workflow Sample Location

The workflow sample "Quality Filter" can be found in the "Custom Elements" section of the Workflow Designer samples.

Workflow Image

The workflow looks as follows:

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  <img src="/wiki/download/attachments/16122711/Quality Filter.png"/>
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Workflow Wizard

The wizard has 2 pages.

  1. Input sequence(s): On this page you must input sequence(s). 

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      <img src="/wiki/download/attachments/16122711/Quality Filter_1.png"/>
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  2. Quality Filter: On this page you can modify quality filter and output settings. 

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      <img src="/wiki/download/attachments/16122711/Quality Filter_2.png"/>
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    The following parameters are available:

    Minimum quality valueMinimum quality value
    Result FASTQ fileLocation of output data file. If this attribute is set, slot "Location" in port will not be used.
    Accumulate resultsAccumulate all incoming data in one file or create separate files for each input.In the latter case, an incremental numerical suffix is added to the file name.