When you open UGENE for the first time, there is an empty UGENE window with the main menu bar. Using these menus you can run many algorithms, configure different settings and get help.

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You can select and analyze different files such as sequences, multiple sequence alignments, short reads assemblies and etc. To open a file click on the File-->Open or click on the Open icon and choose the file:

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1. <B>Nucleotide or protein sequence</B> If you choose a file with a nucleic or protein sequence you can see the following picture with the project view with your document, sequence view with your sequence and its annotations, options panel with many available options. You can also use algorithms and instruments from the context menu: 

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  <img src="/wiki/download/attachments/2523429/pic4_1.png"/>
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Example 1: For example, you want to find open reading frames (ORFs) in your sequence. For this select the Analyze-->Find ORFs item from the context menu:

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  <img src="/wiki/download/attachments/2523429/pic6.png"/>
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or by the toolbar Show ORFs icon:

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After that you will see your sequence with ORFs:

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  <img src="/wiki/download/attachments/2523429/pic8_1.png"/>
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2. If you open a file with a multiple sequence alignment you can see the following picture where there are Options Panel, Toolbar and context menu with many instruments and algorithms:

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  <img src="/wiki/download/attachments/2523429/pic5.png"/>
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Example 2: For example, you may want to build a tree from your alignment. You can do this by three different ways:

a. From the toolbar:

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  <img src="/wiki/download/attachments/2523429/pic9.png"/>
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b. From the context menu:

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  <img src="/wiki/download/attachments/2523429/pic10.png"/>
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c. From the Options Panel:

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  <img src="/wiki/download/attachments/2523429/pic11.png"/>
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When you choose the building tree parameters in the dialogs the tree appears:

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  <img src="/wiki/download/attachments/2523429/pic12.png"/>
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3. If you click a file a short reads assembly, like BAM or SAM format you see the following picture:

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  <img src="/wiki/download/attachments/2523429/pic13.png"/>
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Example 3: For example, you want to highlight the strand direction reads. You can do this by two ways: by the context menu or by the Options Panel menu.

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  <img src="/wiki/download/attachments/2523429/pic14 (3).png"/>
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These were the basic functions provided for you by UGENE. Read more about advanced options in the next chapter.