Prepare a query schema and save it to a file.

Open a nucleotide sequence that you want to analyze with this query schema. You can see the sequence displayed in the Sequence view.

To learn more about the Sequence view read the main UGENE User Manual.

Select the Analyze ‣ Analyze with Query Schema item in the Actions main menu or in the context menu:

<center>
     <br>
     <img src="/wiki/download/attachments/16122046/Running Schema from the Sequence View.png"/>
     <br>
</center>

The Analyze with query schema dialog appears:

<center>
     <br>
     <img src="/wiki/download/attachments/16122046/Running Schema from the Sequence View_1.png"/>
     <br>
</center>

Browse for the file with a query schema. The selected schema preview appears in the dialog, for example:

<center>
     <br>
     <img src="/wiki/download/attachments/16122046/Running Schema from the Sequence View_2.png"/>
     <br>
</center>

You can also adjust other parameters:

Region — the sequence range to analyze with the query schema, you can select:

In the Save annotation(s) to group you can set up a file to store annotations.  It could be either an existing annotation table object, a new annotation table or auto-annotations table (if it is available).

In the Annotation parameters group you can specify the name of the group. If the group name is set to <auto> UGENE will use the group name as the name for the group. Also you can add a description in the corresponding text field.