To load sequences to ExpertDiscovery click on the New ExpertDiscovery Document toolbar button:

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The following dialog will appear:

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Load the sequences you want to analyze by choosing any file with a sequence or multiple sequences. Positive sequence base contains a regulation object you are interested in. Negative sequence base doesn't have it. You also may generate negative sequences automatically. ExpertDiscovery will extract complex signals which reflect a structure of your regulation object. The more sequences you provide the better will be result.