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Scans each input sequence from the end to find the first position where the quality is greater or equal to the minimum quality threshold.

Then it trims the sequence to that position.

If a whole sequence has quality less than the threshold or the length of the output sequence less than the minimum length threshold then the sequence is skipped.

Elemet type: SequenceQualityTrim

Parameters

ParameterDescriptionDefault valueParameter in Workflow FileType
Trimming quality threshold

Quality threshold for trimming.

30qual-idnumeric
Min length

Too short reads are discarded by the filter.

0len-idnumeric
Trim both ends

Trim both ends of a read or not. Usually, you need to set True for Sanger sequencing and False for NGS

Trueboth-endsboolean

Input/Output Ports

The element has 1 input port. 

Name in GUI: Input data

Name in Workflow File: in-sequence

Slots:

Slot In GUISlot in Workflow FileType
Sequencesequencesequence

 And 1 output port:

Name in GUI: Output data

Name in Workflow File: out-sequence

Slots:

Slot In GUISlot in Workflow FileType
Sequencesequencesequence
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