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Finds annotations for DNA sequence in a local BLAST database.

Element type: blast-plus

BLAST+ is used as an external tool from UGENE and it must be installed on your system. To learn more about the external tools, please, read main UGENE User Manual.


ParameterDescriptionDefault valueParameter in Workflow FileType
Search typeSelects the type of the BLAST searches.blastnblast-type


Available values are:

  • blastn
  • blastp
  • blastx
  • tblastn
  • tblastx
Database pathPath to the database files.
Database nameBase name for BLAST DB files.
Tool path Path to the BLAST executable.defaulttool-pathstring
Temporary directoryDirectory for temporary files.defaulttemp-dirstring
Expected valueExpectation threshold value.10e-valnumeric
Culling limitIf the query range of a hit is enveloped by that of at least this many higher-scoring hits, delete the hit0max-hitsnumeric
Annotate asName of the result annotations.blast_resultresult-namestring
Gapped alignmentPerform gapped alignment.usegapped-alnboolean
Gap costsCost to create and extend a gap in an alignment.2 2gap-costsstring
Match scoresReward and penalty for matching and mismatching bases.1 -3match-scoresstring
BLAST outputLocation of BLAST output file.
BLAST output typeType of BLAST output file.XML (-outfmt 5)type-outputstring

Input/Output Ports

The element has 1 input port:

Name in GUI: Input sequence

Name in Workflow File: in-sequence


Slot In GUISlot in Workflow FileType

And 1 output port:

Name in GUI: Annotations

Name in Workflow File: out-annotations


Slot In GUISlot in Workflow FileType
Set of annotationsannotationsannotation-table
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