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The workflow scans each input sequence from the end to find the first position where the quality is greater or equal to the minimum quality threshold. Then it trims the sequence to that position. If a the whole sequence has quality less than the threshold or the length of the output sequence less than the minimum length threshold then the sequence is skipped.

Element type: QualityTrim

Parameters

ParameterDescriptionDefault valueParameter in Workflow FileType
Output directorySelect an output directory. Custom - specify the output directory in the 'Custom directory' parameter. Workflow - internal workflow directory. Input file - the directory of the input file.Input fileout-modenumeric
Custom directory

Specify the output directory.


custom-dirstring
Output file nameA name of an output file. If default of empty value is provided the output name is the name of the first file with additional extention.
out-namestring
Quality thresholdQuality threshold for trimming.30qual-idnumeric
Min LengthToo short reads are discarded by the filter.0len-idnumeric
Trim both endsTrim the both ends of a read or not. Usually, you need to set True for Sanger sequencing and False for NGSTrueboth-endsboolean

Input/Output Ports

The element has 1 input port:

Name in GUI: Input File

Name in Workflow File: in-file

Slots:

Slot In GUISlot in Workflow FileType
Source URLurlstring

And 1 output port:

Name in GUI: Output File

Name in Workflow File: out-file

Slots:

Slot In GUISlot in Workflow FileType
Source URLurlstring
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