Reads in FASTQ file produced by CASAVA 1.8 contain 'N' or 'Y' as a part of an idetifier. 'Y' if a read if filtered, 'N' if the read if the read is not filtered. The workflow cleans up the filtered reads. For example:
@HWI-ST880:181:D1WRUACXX:8:1102:4905:2125 1:N:0:TAAGGG CTTACATAACTACTGACCATGCTCTCTCTTGTCTGTCTCTTATACACATCT + 111442222322324232AAFFHIJJJJJJIHIIF111CGGFHIG???FGB @HWI-ST880:181:D1WRUACXX:8:1102:7303:2101 1:Y:0:TAAGGG TCCTTACTGTCTGAGCAATGGGATTCCATCTTTTACGATCTAGACATGGCT + 11++4222322.
Parameters in GUI
Parameter | Description | Default value |
---|---|---|
Output directory | Select an output directory. Custom - specify the output directory in the 'Custom directory' parameter. Workflow - internal workflow directory. Input file - the directory of the input file. | Input file |
Custom directory | Specify the output directory. | |
Output file name | A name of an output file. If default of empty value is provided the output name is the name of the first file with additional extention. |
Parameters in Workflow File
Type: CASAVAFilter
Parameter | Parameter in the GUI | Type |
---|---|---|
out-mode | Output directory | numeric |
custom-dir | Custom directory | string |
out-name | Output file name | string |
Input/Output Ports
The element has 1 input port:
Name in GUI: Input File
Name in Workflow File: in-file
Slots:
Slot In GUI | Slot in Workflow File | Type |
---|---|---|
Source URL | url | string |
And 1 output port:
Name in GUI: Output File
Name in Workflow File: out-file
Slots:
Slot In GUI | Slot in Workflow File | Type |
---|---|---|
Source URL | url | string |