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  • Highlighting Alignment

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  • Agreements — .highlights symbols that coincide with the reference sequence. 

  • Disagreements — highlights nucleotides that differ from the reference sequence.

  • Gaps - highlights gaps.
  • Transitions - highlights transitions.
  • Transversions - highlights transversions.

To use dots instead of symbols which are not highlighted check the Use dots checkbox in the Options Panel or use the Highlighting->Use dots context menu item.

To select a reference sequence use the Set this sequence as reference context menu or Reference sequence field in the Highlighting tab of the Options Panel.

 Also you can export highlighting with a help of the Export button in the Options Panel.