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Reverse primer - on the opposite strand from the forward primer. The primer length should be between 15 and 50 bases.
Note: you should take in account, that algorithm calculates no more than 50 forward and 50 reverse primers, which means, that you can take 50x50=2500 products maximum.
Mismatches - mismatches limit.
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Maximum product - the maximum size of the amplified sequence. You should take in account, that algorithm calculates no more than 50 forward and 50 reverse primers, which means, that you can take 50x50=2500 products maximum. Use ambiguous bases - search for ambiguous bases (as \"N\") if checked.
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