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Unipro UGENE User Manual
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Unipro UGENE User Manual
User Manual
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Extensions
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Created by
Yuliya Algaer
on
Jun 02, 2021
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DNA Annotator
Find Group of Annotated Regions
Plasmid Auto Annotation
DNA Flexibility
Configuring Dialog Settings
Result Annotations
DNA Statistics
DNA Generator
ORF Marker
Remote BLAST
Primer-BLAST
Exporting BLAST Results to Alignment
Fetching Sequences from Remote Database
BLAST
Creating Database
Making Request to Database
Fetching Sequences from Local BLAST Database
Repeat Finder
Repeats Finding
Tandem Repeats Finding
Tandem Repeats Search Result
Restriction Analysis
Selecting Restriction Enzymes
Using Custom File with Enzymes
Filtering by Number of Hits
Excluding Region
Circular Molecule
Results
Molecular Cloning in silico
Digesting into Fragments
Creating Fragment
Constructing Molecule
Available Fragments
Fragments of the New Molecule
Changing Fragments Order in the New Molecule
Removing Fragment from the New Molecule
Editing Fragment Overhangs
Adjusting fragment ends
Reverse Complement a Fragment
Other Constuction Options
Output
Creating PCR Product
In Silico PCR
Primers Details
Primer Library
PCR Primer Design for DNA Assembly
Backbone details
Secondary Structure Prediction
SITECON
SITECON Searching Transcription Factors Binding Sites
Types of SITECON Models
Eukaryotic
Prokaryotic
Building SITECON Model
Smith-Waterman Search
HMM2
Building HMM2 Model
Calibrating HMM2 Model
Searching Sequence Using HMM2 Profile
HMM3
Building HMM Model
Searching Sequence Using HMM Profile
Searching Sequence Against Sequence Database
uMUSCLE
MUSCLE Aligning
Aligning Profile to Profile with MUSCLE
Aligning Sequences to Profile with MUSCLE
ClustalW
MAFFT
T-Coffee
Bowtie
Bowtie Aligning Short Reads
Building Index for Bowtie
Bowtie 2
Bowtie 2 Aligning Short Reads
Building Index for Bowtie 2
BWA
Aligning Short Reads with BWA
Building Index for BWA
BWA-SW
Aligning Short Reads with BWA-SW
Building Index for BWA-SW
BWA-MEM
Aligning Short Reads with BWA-MEM
Building Index for BWA-MEM
UGENE Genome Aligner
Building Index for UGENE Genome Aligner
Converting UGENE Assembly Database to SAM Format
Map NGS Reads with UGENE Genome Aligner
CAP3
SPAdes
Weight Matrix
Searching JASPAR Database
Building New Matrix
Primer3
Primer3 (no target sequence)
Posterior Actions
RTPCR Primer Design
Spliced Alignment mRNA and cDNA
External Tools Plugin
Configuring External Tool
ClustalO
Kalign Aligning
GeneCut desktop
mfold
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Overview
Content Tools
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