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- Suppliers - check/uncheck suppliers, whose enzymes will be shown. By default, enzymes with undefined commercial suppliers are not shown.
- Recognition sequence length - show only enzymes, whose recognition sequence length is in bounds. NOTE: N bases are not included in recognition site. That means, that if enzyme has the following sequence:
C C N N N N N N N G G
its recognition site length will be four, not eleven. - Overhang type - show only enzymes with certain overhang type. The following options are presented:
- Any - show all enzymes.
- No overhang - site has no cuts at all. Example:
3' CTCGAG 5'
5' GAGCTC 3' - 5' - the cut of the forward strand is to the left of the cut of the reverse-complementary strand. Example:
3' C C▼T N A G G 5'
5' G G A N T▲C C 3' - 3' - the cut of the forward strand is to the right of the cut of the reverse-complementary strand. Example:
3' C G A T▼C G 5'
5' G C▲T A G C 3' - Blunt - the cut is cuts are located in the middle of the site.
- Sticky - the cut is cuts are locate anywhere but the middle of the site. It is 5' and 3' both.
- Nondegenerate sticky - the same as Sticky, but the overhang between cuts has only A, C, G or T (no extended) symbols.
- Blunt or sticky - Blunt + Sticky.
- Blunt or nondegenerate sticky - Blunt + Nondegenerate sticky.
- Keep only - show most interesting enzymes only:
- Palindromic - forward and reverse-complementary strands are equal.
- Uninterrupted - no internal N bases.
- Nondegenerate - No extended DNA alphabet symbols (only A, C, G, T and N).
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