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 When you open UGENE for the first time, there is an empty UGENE window with the main menu bar. Using these menus you can run many algorithms, configure different settings and get help.

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Now you can select and analyze different files such as sequences, multiple sequence alignments, short reads assemblies and etc. To open a file click to the File-->Open or click to the Open icon and choose the file:

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or by the toolbar Show ORFs icon:

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When you choose the building tree parameters in the dialogs the tree appears:

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