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3' perfect match - specify the number of nucleotides at the 3' end that must not have mismatches.
Maximum product size - the maximum size of the amplified sequence. Use ambiguous bases - search for ambiguous bases (as \"N\") if checked.
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3' perfect match - specify the number of nucleotides at the 3' end that must not have mismatches.
Maximum product size - the maximum size of the amplified sequence. Use ambiguous bases - search for ambiguous bases (as \"N\") if checked.
...