Some mutations could have a chromatogram signal lesser than the reference one. This situation very often appears if the mix of cells (healthy and oncological) has been being analyzed. That means that the mutation signal could be different (due to a different concentration of oncological cells in a sample).
These kinds of mutations we call Alternative mutations and very often it's important to visualize them clearlyLook at the picture below. The signal of the "C" nucleotide (blue) is the highest. But there is a signal of the "T" nucleotide (red), which is high enough too. That is the thing we call Alternative mutation.
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