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- Instead of "Save annotation(s) to" and "Annotations paramters" settings you can see a list of selected primer pairs. That means, that BLAST will be run three times for three primer pairs - pair 1, pair 4 and pair 3. All results will be stored to the annotation group with a primer pair, which was an input for this calculation.
- The "Megablast" option is enabled by default. Disable this option to get more results (it affects calculation time).
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