UGENE has certain primers format. It allows one to use some features, which are avaliable only if this format is being recognized. Examples of thes features:
- Create PCR Fragment,
- Primer-BLAST.
If you have a primer pair, which doesn't fit to this format, these features will be unavaliable for you. To fix this issue:
- Select your own left and right primers.
- Click right mouse button → Edit → Transform into a primer pair.
After these steps are performed, a new primer pair, fit to UGENE format, will appear: -
