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Multiple sequence alignment (MSA Editor). The Alignment Editor is a powerful tool for visualization and editing DNA, RNA or protein multiple sequence alignments. To activate the Alignment Editor open any alignment file. For example you can use the $ugene/data/samples/CLUSTALW/COI.aln file provided with UGENE. After opening the file in UGENE the Alignment Editor window appears:



The editor supports different multiple sequence alignment (MSA) formats, such as ClustalW, MSF and Stockholm. The editor provides interactive visual representation which includes:

  • Navigation through an alignment;
  • Optional coloring schemes (for example Clustal, Jalview like, etc.);
  • Flexible zooming for large alignments;
  • Export publication-ready images of alignment;
  • Several consensus calculation algorithms.

Using the Alignment Editor you can:

  • Perform multiple sequence alignment using integrated MUSCLE and KAlign algorithms;
  • Edit an alignment: delete/copy/paste symbols, sequences and subalignments;
  • Build phylogenetic trees;
  • Generate grid profiles;
  • Build Hidden Markov Model profiles to use with HMM2/HMM3 tools.

Example 2Build a tree from your alignment. You can do this by three different ways:

a. From the toolbar. Click to the tree icon:



b. From the context menu:



c. From the Options Panel:



After calculation the tree appears in the MSA Editor in a separate window:



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